Количественная оценка стиму-ляции коллагеногенеза пласти-ческими материалами

Авторы: Беланов Г.Н., Байриков И.М., Волова Л.Т., Колсанов А.В., Толстов А.В.

RUSSIAN FEDERATION FEDERAL SERVICE FOR INTELLECTUAL PROPERTY, PATENTS AND TRADEMARKS (19)

RU(11) 2 281 513(13) C2 (51) Int. Cl. G01N 33/68 (2006.01) C12N 5/08 (2006.01) A61K 35/36 (2006.01) (12)

ABSTRACT OF INVENTION (21), (22)

Application: 2004128368/15, 23.09.2004 (24)

Effective date for property rights: 23.09.2004 (43)

Application published: 10.03.2006 (45) Date of publication: 10.08.2006 Bull. 22

Mail address: 443100, g.Samara, ul. Molodogvardejskaja, 225, kv.148, V.G. Podkovkinu (72)

Inventor(s): Volova Larisa Teodorovna (RU), Podkovkin Vladimir Georgievich (RU), Rossinskaja Viktorija Viktorovna (RU) (73) Proprietor(s): Volova Larisa Teodorovna (RU), Podkovkin Vladimir Georgievich (RU), Rossinskaja Viktorija Viktorovna (RU) (54)

METHOD FOR QUANTITATIVE EVALUATION OF STIMULATED COLLAGENOGENESIS WITH BIOPLASTIC MATERIALS (57)

Abstract: FIELD: medicine.

SUBSTANCE: one should place bioplastic material in culture liquid with a culture of dermal fibroblasts to cultivate fibroblasts together with bioplastic material for 4 d, then it is necessary to sample culture liquid and detect the concentration of protein-bound hydroxyproline. One concludes upon the degree of collagenogenesis stimulation with tested bioplastic material by greater or lesser degree of increased concentration of protein-bound hydroxyproline in sampled culture liquid against the control. As a control sample it is useful to apply culture liquid in which one could observe the tested bioplastic material for 4 d but no fibroblasts. The innovation is simple and enables to quantitatively evaluate the stimulation of collagenogenesis with bioplastic materials without applying any laboratory animals.

EFFECT: higher accuracy of evaluation.

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